module MiGA::Cli::Action::Doctor::Operations

Public Instance Methods

check_cds(cli) click to toggle source

Perform cds operation with MiGA::Cli cli

# File lib/miga/cli/action/doctor/operations.rb, line 70
def check_cds(cli)
  cli.say 'Looking for unzipped genes or proteins'
  n, k = cli.load_project.dataset_names.size, 0
  cli.load_project.each_dataset do |d|
    cli.advance('Datasets:', k += 1, n, false)
    res = d.result(:cds) or next
    changed = false
    %[genes proteins gff3 gff2 tab].each do |f|
      file = res.file_path(f) or next
      if file !~ /\.gz/
        cli.say "  > Gzipping #{d.name} #{f}   "
        run_cmd(['gzip', '-9', file])
        changed = true
      end
    end
    if changed
      d.add_result(:cds, true, force: true)
      sr = d.result(:stats) and sr.remove!
    end
  end
  cli.say
end
check_ess(cli) click to toggle source

Perform essential-genes operation with MiGA::Cli cli

# File lib/miga/cli/action/doctor/operations.rb, line 95
def check_ess(cli)
  cli.say 'Looking for outdated essential genes'
  cli.load_project.each_dataset do |d|
    res = d.result(:essential_genes)
    next if res.nil?

    dir = res.file_path(:collection)
    if dir.nil? || outdated_fastaai_ess(res)
      cli.say "  > Removing #{d.name}:essential_genes"
      res.remove!
      d.result(:stats)&.remove!
      next
    end
    next if Dir["#{dir}/*.faa"].empty?

    cli.say "  > Fixing #{d.name}"
    run_cmd <<~CMD
      cd #{dir.shellescape} && tar -zcf proteins.tar.gz *.faa && rm *.faa
    CMD
  end
end
check_files(cli) click to toggle source

Perform files operation with MiGA::Cli cli

# File lib/miga/cli/action/doctor/operations.rb, line 45
def check_files(cli)
  cli.say 'Looking for outdated files in results'
  n, k = cli.load_project.dataset_names.size, 0
  cli.load_project.each_dataset do |d|
    cli.advance('Datasets:', k += 1, n, false)
    d.each_result do |r_k, r|
      ok = true
      r.each_file do |_f_sym, _f_rel, f_abs|
        unless File.exist? f_abs
          ok = false
          break
        end
      end
      unless ok
        cli.say "  > Registering again #{d.name}:#{r_k}   "
        d.add_result(r_k, true, force: true)
        d.result(:stats)&.remove!
      end
    end
  end
  cli.say
end
check_mts(cli) click to toggle source

Perform mytaxa-scan operation with MiGA::Cli cli

# File lib/miga/cli/action/doctor/operations.rb, line 119
def check_mts(cli)
  cli.say 'Looking for unarchived MyTaxa Scan runs'
  cli.load_project.each_dataset do |d|
    res = d.result(:mytaxa_scan)
    next if res.nil?

    dir = res.file_path(:regions)
    fix = false
    unless dir.nil?
      if Dir.exist? dir
        run_cmd <<~CMD
          cd #{dir.shellescape}/.. \
              && tar -zcf '#{d.name}.reg.tar.gz' '#{d.name}.reg'                  && rm -r '#{d.name}.reg'
        CMD
      end
      fix = true
    end
    %[blast mytaxain wintax gene_ids region_ids].each do |ext|
      file = res.file_path(ext)
      unless file.nil?
        FileUtils.rm(file) if File.exist? file
        fix = true
      end
    end
    if fix
      cli.say "  > Fixing #{d.name}"
      d.add_result(:mytaxa_scan, true, force: true)
    end
  end
end
check_refdb(cli) click to toggle source

Perform refdb operation with MiGA::Cli cli

# File lib/miga/cli/action/doctor/operations.rb, line 5
def check_refdb(cli)
  cli.say 'Checking index format of reference database'
  ref_dbs = File.join(ENV['MIGA_HOME'], '.miga_db')
  manif_file = File.join(ref_dbs, '_local_manif.json')
  return unless File.size?(manif_file)

  MiGA::Json.parse(manif_file)[:databases]&.keys&.each do |db|
    p = MiGA::Project.load(File.join(ref_dbs, db.to_s))
    md = p&.metadata
  end
end
check_start(cli) click to toggle source

Perform start operation with MiGA::Cli cli

# File lib/miga/cli/action/doctor/operations.rb, line 153
def check_start(cli)
  cli.say 'Looking for legacy .start files lingering'
  cli.load_project.each_dataset do |d|
    d.each_result do |r_k, r|
      if File.exist? r.path(:start)
        cli.say "  > Registering again #{d.name}:#{r_k}"
        r.save
      end
    end
  end
end
check_status(cli) click to toggle source

Perform status operation with MiGA::Cli cli

# File lib/miga/cli/action/doctor/operations.rb, line 19
def check_status(cli)
  cli.say 'Updating metadata status'
  p = cli.load_project
  n = p.dataset_names.size
  (0 .. cli[:threads] - 1).map do |i|
    Process.fork do
      k = 0
      cli.load_project.each_dataset do |d|
        k += 1
        cli.advance('Datasets:', k, n, false) if i == 0
        d.recalculate_status if k % cli[:threads] == i
      end
    end
  end
  Process.waitall
  cli.say
end
check_tax(cli) click to toggle source

Perform taxonomy operation with MiGA::Cli cli

# File lib/miga/cli/action/doctor/operations.rb, line 167
def check_tax(cli)
  # cli.say 'o Checking for taxonomy/distances consistency'
  # TODO: Find 95%ANI clusters with entries from different species
  # TODO: Find different 95%ANI clusters with genomes from the same species
  # TODO: Find AAI values too high or too low for each LCA rank
end