ROCker employs Receiver-Operator Curve (ROC)-analysis of in silico generated metagenomic
reads that map onto well-curated reference protein sequences, and a sliding window across the length
of the reference sequences to deal with non-discriminative domains and motifs that are shared between
functionally different proteins.
You can download stand-alone ROCker. If you use ROCker, please cite:
- Orellana LH, Rodriguez-R LM & Konstantinidis KT (under review). Detecting and quantifying functional genes in short-read metagenomic datasets: method development and application to the nitrogen cycle genes.