The ANI calculator estimates the average nucleotide identity using both best hits (one-way ANI) and
reciprocal best hits (two-way ANI) between two genomic datasets, as calculated by
Goris et al., 2007.
Typically, the ANI values between genomes of the same species are above 95% (e.g.,
Escherichia coli). Values below 75% are not to be
trusted, and AAI
should be used instead. This tool supports both complete and draft genomes (multi-fasta).
For an introductory comment, see
Rodriguez-R & Konstantinidis, 2014.
Escherichia vs Yersinia,
Escherichia vs Xanthomonas.