FastQ.test-error.rb
Compares the estimated error of sequencing reads (Q-score) with observed mismatches (identity against a know reference sequence).
See source code, Artistic license 2.0.
§ References
Rodriguez-R & Konstantinidis, 2016, PeerJ Preprints.
§ Requirements
§ Usage
FastQ.test-error.rb --fastq in_file --blast in_file --out out_file [opts]
§ Arguments
- FastQ*
--fastq in_file
FastQ file containing the sequences.- Tabular BLAST*
--blast in_file
Tabular BLAST file mapping reads to reference sequences.- Output*
--out out_file
Output tab-delimited file to create.- Quiet
--quiet
Run quietly (no STDERR output).